Studies on the immunological interactions between human mammary tumor associated glycoproteins and their monoclonal antibody by electrochemical impedance immunoanalytical method

An electrochemical impedance method has been developed for the specific det ection of immunological interactions between human mammary tumor associated glycoproteins and its monoclonal antibody (GP1D8). Antibody proteins were immobilized through oriented covalent coupling onto AT - cut 9MHz quartz cr ystals microbalance(QCM) transducer. The experimental set - up for studying the immunological response consists of a differential measurement system t hat allows extraction of only the immunological signal. Consequently, measu rements of the electrochemical impedance spectra(EIS) of gold electrode coa ted with the antibody showed changes of the a.c. current response after the addition of the specific antigen. The successful immunological reaction be tween the immobilized antibody - antigen could thus be monitored.