Purification, crystallization and X-ray analysis of crystals of pectate lyase A from Erwinia chrysanthemi

Authors
Doan, CN Caughron, MK Myers, JC Breakfield, NW Oliver, RL Yoder, MD
Citation
Cn. Doan et al., Purification, crystallization and X-ray analysis of crystals of pectate lyase A from Erwinia chrysanthemi, ACT CRYST D, 56, 2000, pp. 351-353
Citations number
19
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
56
Year of publication
2000
Part
3
Pages
351 - 353
Database
ISI
SICI code
0907-4449(200003)56:<351:PCAXAO>2.0.ZU;2-W
Abstract
Pectate lyase A is secreted by Erwinia chrysanthemi and is a virulence fact or for soft rot diseases in plants. Crystals of pectate lyase A were obtain ed by vapor-diffusion techniques in the presence of polyethylene glycol. Th e crystals belong to the monoclinic space group P2(1), with unit-cell param eters a = 48.96, b = 148.86, c = 78.61 Angstrom, beta = 97.32 degrees. The crystals contain two protein molecules of 38 kDa per asymmetric unit and di ffract to 2.11 Angstrom using Ca K alpha radiation.