Neonatal pancreas transplantation and liver enzyme activities in diabetic mice

The aim of this study was to estimate the influence of transplantation of n eonatal pancreas from singenic donors on glucose production and utilisation in alloxan diabetic mice. The alloxan diabetic mice, 20 days after alloxan, received neonatal pancrea ses from singenic donors under the kidney capsule. Enzymes involved in glyc olytic, gluconeogenic, lipogenic and pentose phosphate pathway were examine d in liver of experimental mice. The fructose-1,6-diphosphatase (FDPase) activity in the liver of diabetics increased for about 60%, while the pyruvate kinase (PK) and ATP-citrate lya se (CCE) activity decreased for about 40% of the values in the healthy mice . The values of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluc onate dehydrogenase (6-PGDH) were not statistically different in diabetic l iver compared with the liver of healthy animals. After pancreas transplanta tion some of diabetic animals become normoglycaemic, while the others remai ned hyperglycaemic. The FDPase activity in hyperglycaemic diabetic recipien ts was similar to those in diabetic mice that did not received transplant, while in normoglycaemic recipients the FDPase activity returned to the norm al values. However, the activities of PK and CCE in hyperglycaemic recipien ts were similar to those in healthy animals, while in normoglycaemic recipi ents the enzyme activities were much higher. Results obtained showed that glycolytic, gluconeogenic and lipogenic enzyme activities in the liver of normoglycaemic mice that received pancreas tran splant reached approximate the physiological values within 30 days after tr ansplantation.