B. Schmidt et al., Lobular distribution pattern of lactate dehydrogenase and 6-phosphogluconate dehydrogenase activity in rat liver, ACT HISTOCH, 102(1), 2000, pp. 37-47
Lactate dehydrogenase (LDH) and 6-phosphogluconate dehydrogenase (6-PGDH) a
ctivities were measured in lobular areas expanding between 3 portal tracts
and an efferent central vein in the livers of male Wistar rats, using a Low
ry technique. The maximum of LDH activity was found in a nearly uniform bro
ad area in the lobular periphery. From that area values decreased along per
iportal/septal -> perivenous gradients, but only slightly within that area
along the periportal -> septal axis of the vascular septum. Maximum values
of 6-PGDH activity were present in an intermediate area close to the centra
l vein demonstrating a rather inhomogeneous distribution pattern without a
clear definition of zonal limits. Our data on the distribution pattern of L
DH are in agreement with the concept of the metabolic lobulus and are suppo
rted by a recent evaluation of the vascular architecture in rat liver. The
lobular distribution pattern of 6-PGDH cannot be interpreted without doubt
in accordance with that concept.