Effects of 2-[3-estrone-N-ethyl-piperazine-methyl]tetracycline (XW630) on osteoporosis in ovariectomized rats

AIM: To study the effects of 2-[3-estrone-N-ethylpiperazine-methyl]tetracyc line (XW630) on experimental osteoporosis in ovariectomized (OVX) rats. MET HODS: Serum estradiol (E-2) content and bone l-carboxyglutamic acid-contain ing protein (BGP) content were measured by radioimmunoassay. With undecalci fied bone section and tetracycline intraperitoneal labeling, the bone stati c and dynamic data were studied in right femur samples. RESULTS: After trea tment with XW630 2.5 mg.kg(-1), serum BGP content increased by 75.7% but th ere was no change in serum E-2 content and uterus weight compared with OVX rats. Compared with OVX rats, the static data of trabecular bone volume/tot al tissue volume, trabecular bone volume/sponge bone volume, and mean trabe cular plate density were enhanced after treatment with XW630 for 13 wk, The dynamic data of single-labeled surface, double-labeled surface, trabecular osteoid surface, and bone formation rate in tissue level in XW630 group we re increased and osteoid maturation period was shortened. CONCLUSION: XW630 enhanced bone activation frequency add increased trabecular connectivity, stability, and strength. XW630 stimulated bone formation and inhibited bone resorption with no effeet on reproductive system.