DETERMINATION OF GLUCOSE BY MEANS OF AN E NZYMATIC METHOD

In this work a reliable method has been worked out for the enzymatic d etermination of glucose. For the measurement of samples a reagent cons isting of glucose oxidase (GOD), peroxidase (POD) and o-dianisidine (O DN) was prepared. The change in colour intensity of the reactions mixt ure was recorded spectrophotochemically at 420 nm. The accuracy of the method was checked by comparison with the Boehringer Test. Applicatio n of the method: 1. laboratory-scale and industrial-scale measurement of glucoamylase and trans-glucosidase activity; 2. hydrolysis of biode gradable films; 3. following the process of cornstarch saccharificatio n; 4. genetic measurements.