Altered L-type Ca2+ channel currents in vascular smooth muscle cells from experimental diabetic rats

Vascular complications of diabetes are associated with abnormal Ca2+ handli ng by vascular smooth muscle cells (SMCs) in which the alteration in L-type voltage-dependent Ca2+ channel (VDCC) currents may play an important role. In the present study, the characteristics of L-type VDCC currents in tail artery SMCs from streptozotocin-induced diabetic rats were examined. The de nsities, but not the voltage dependence, of L-type VDCC currents were reduc ed as diabetes progressed from 1 wk to 3 mo. The inhibitory effect of dibut yryl-cAMP on L-type VDCC currents was greater in diabetic SMCs than in age- matched control cells (P < 0.01). Both the stimulatory effect of BAY K 8644 and the inhibitory effect of nifedipine on L-type VDCC currents were signi ficantly enhanced in diabetic cells. The diabetes-related abnormalities in L-type VDCC currents were mimicked by culturing SMCs with a high concentrat ion of glucose. Our results suggest that the properties of L-type VDCC in d iabetic vascular SMCs were significantly altered, partially related to the increased L-type VDCC sensitivity to cAMP and hyperglycemia.