Assignment of overlapping H-1 NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy

Authors
Kalic, M Lauterwein, J Busselmann, G Kamps, G
Citation
M. Kalic et al., Assignment of overlapping H-1 NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy, BIOC BIOP R, 269(1), 2000, pp. 76-80
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
269
Issue
1
Year of publication
2000
Pages
76 - 80
Database
ISI
SICI code
0006-291X(20000305)269:1<76:AOOHNS>2.0.ZU;2-3
Abstract
The H-1 NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal id entification of proline, glutamate, taurine, and methionine sulfoxide, alth ough several hey proton signals were strongly overlapped. (C) 2000 Academic Press.