Phage display of RNase A and an improved method for purification of phagesdisplaying RNases

Authors
Korn, K Foerster, HH Hahn, U
Citation
K. Korn et al., Phage display of RNase A and an improved method for purification of phagesdisplaying RNases, BIOL CHEM, 381(2), 2000, pp. 179-181
Citations number
12
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOLOGICAL CHEMISTRY
ISSN journal
14316730 → ACNP
Volume
381
Issue
2
Year of publication
2000
Pages
179 - 181
Database
ISI
SICI code
1431-6730(200002)381:2<179:PDORAA>2.0.ZU;2-1
Abstract
Functional ribonuclease A was presented on the surface of the filamentous p hage M13 by fusion to the minor coat protein. RNase activity of the fusion protein was shown by a zymogram assay. In addition, we established a modifi ed method for preparing RNase-displaying phages without contaminating host RNases.