Kinetics of cassava starch hydrolysis with Termamyl (R) enzyme

Kinetic properties of Termamyl(R) 120L were investigated with respect to st arch hydrolysis in a batch reactor at substrate concentrations of 50-270 g. dm(-3) and enzyme concentrations of 0.17-1 cm(3).dm(-3) (i.e., cm(3) of Nov o Nordisk enzymatic solution per dm(3) of raw cassava starch suspension). A general kinetic expression giving product concentration as a function of t ime was first developed; an equation relating the reaction rate of each pro duct to the sum of the concentrations of oligosaccharides with a higher deg ree of polymerisation was then derived. The empirical model satisfactorily fits experimental data for oligosaccharides with a degree of polymerization ranging from 1 to 7. (C) 2000 John Wiley & Sons, Inc.