Wgj. Degen et al., The fate of U1 snRNP during anti-Fas induced apoptosis: specific cleavage of the U1 snRNA molecule, CELL DEAT D, 7(1), 2000, pp. 70-79
During apoptosis, the U1-70K protein, a component of the spliceosomal U1 sn
RNP complex, is specifically cleaved by the enzyme caspase-3, converting it
into a C-terminally truncated 40-kDa fragment. In this study, we show that
the 40-kDa U1-70K fragment is still associated with the complete U1 snRNP
complex, and that no obvious modifications occur with the U1 snRNP associat
ed proteins U1A, U1C and Sm-B/B'. Furthermore, it is described for the firs
t time that the U1 snRNA molecule, which is the backbone of the U1 snRNP co
mplex, is modified during apoptosis by the specific removal of the first 5-
6 nucleotides including the 2,2,7-trimethylguanosine (TMG) cap. The observa
tions that U1 snRNA cleavage is very specific (no such modifications were d
etected for the other U snRNAs tested) and that U1 snRNA cleavage is marked
ly inhibited in the presence of caspase inhibitors, indicate that an apopto
tically activated ribonuclease is responsible for the specific modification
of U1 snRNA during apoptosis.