A multivariate linear model for calculating response feature parameters ofchemical sensor based on fluorescence quenching for analytes

A mathematical model was deduced and described for the different mechanism of fluorescence quenching to analytes based on the essential quenching mode ls including the dynamic quenching, the resonance energy transfer and the i nner filter effects involving absorption of both excited light from the lig ht source and emitted light from membrane, The model reported here indicate d that the logarithmic functions of the intensity of fluorescence modulatio n in virtue of multiple quenching factors conform to the rule of addition. The response feature parameters of chemical sensor based on fluorescence qu enching for analyses could be calculated by combining the multivariate line ar model with a tri-cup method, A conception based on regarding the chemica l sensor as the second source of light was advanced to get more useful info rmation on analytes, The pyrenebutyric acid was chosen as a fluoroprobe for constructing the chemical sensor to response a series of the therapeutic d rugs. A train of thought for constructing the quantitative analysis model f or the chemical membrane sensors based on the fluorescence quenching was af forded in the light of the experimental results.