ESR spin trapping studies on the radicals generated from iron-containing system of xathine oxidase and hypoxathine in the presence of saccharide molecules

The free radicals generated from iron-containing system of xathine oxidase and hypoxathine(Fe-XO/HX) were directly detected by using spin trapping, It was found that not only O-2(-.) and OH . but also alkyl or alkoxyl radical s(R . or RO .) were detected when saccharides such as glucose, fructose and sucrose were added into the Fe-XO/HX system, The amounts of R . or RO . we re increased with the increase of saccharide concentration added to the Fe- XO/HX system. The results showed that saccharide molecules were oxidized by the saccharide-containing Fe-XO/HX system. R . or RO . has a long life tim e and they are liposoluble, Thus, they have more toxicity than hydrophilic O-2(-.) and OH . because the active sites of biomolecules are closely relat ed with lipophilic phase. It is worth mentioning that the results have prov ided an evidence for the hypothesis of "Eating excess sucrose facilitates a ging", The R . or RO . radicals were effectively scavenged by selenium-cont aining abzyme, which displayed a powerful ability to antioxidize.