Electrocatalytic oxidation of NADH at glassy carbon electrodes modified with an electropolymerized film of nile blue A

The kinetic parameters for the electrocatalytic oxidation of dihydronicotia mide adenine dinucleotide (NADH) at glassy carbon electrodes modified with an electropolymerized film of nae blue A (PNB) have been determined based o n rotating disk electrode measurements. The rate constant for the chemical reaction between NADH and PNB is strongly influenced by NADH concentration and the pH value of solution, and it decreases with increasing NADH concent ration, indicating that the electrocatalytic process proceeds via the forma tion of an intermediate of charge-transfer complex between NADH and PNB.