Rapid ("warm") autopsy study for procurement of metastatic prostate cancer

In this report, we describe the distribution of metastases from 14 patients who had hormone-refractory adenocarcinoma of the prostate and agreed while alive to undergo directed autopsies after their deaths. These autopsies we re undertaken specifically to document the distribution of metastases, char acterize tumors phenotypically and immunohistochemically, harvest fresh and snap frozen tumor and normal control tissues suitable for molecular examin ation, and establish cell lines via passages through generations of severe combined immunodeficient and athymic mice, Achievement of these goals was o btained through the development of a multidisciplinary team approach, Team members included a medical oncologist, pathologists, urologists, and resear chers. The autopsy and tissue procurement teams were available on a round-t he-clock basis, The tissues harvested from these autopsies yielded high-qua lity tumor samples, as evidenced by excellent preservation seen by light mi croscopy, strong prostate-specific antigen immunostaining, and the successf ul development of xenografts, The development and expansion of this program represent a valuable resource for molecular and clinical researchers.