Spectral light quality affects protein profile of Synechococcus sp PCC 7942: A comparative 2-dimensional gel electrophoresis (2-DGE) analysis

We report here a comparative analysis of the effect of blue (450 nm), red ( 660 nm), and white light (400-700 nm) on the protein profile of cyanobacter ia Synechococcus sp. PCC 7942. In vivo labeling of cells with [S-35] methio nine and their subsequent analysis by two-dimensional gel electrophoresis ( 2-DGE) showed that eight polypeptides were unique to dark adapted cells, te n were blue light specific, and four were specifically induced in red light . The results show that Synechococcus sp. respond to various light treatmen ts rapidly and synthesize new polypeptides in dark and blue/red light.