Novel chicken CXC and CC chemokines

Upon stimulation with lipopolysaccharide (LPS) the chicken macrophage cell line HD-11 secretes factors with cytokine activity. To characterize these m olecules, representational difference analysis with RNA of LPS-induced and uninduced HD-11 cells was performed, Two cDNA clones were isolated that cod e for polypeptides with structural features of chemokines. cDNA K60 codes f or a novel CXC chemokine of 104 residues including a putative signal peptid e of 20 amino acids at the N-terminus, It is 67% identical to the previousl y cloned chicken chemokine 9E3/CEF4. K60 exhibits a similar degree of seque nce identity to human interleukin 8 and other related CXC chemokines (about 50%), rendering straight-forward predictions of its biological properties difficult. cDNA K203 codes for a novel CC chemokine of 89 amino acids inclu ding a putative N-terminal signal peptide of 21 residues, It is 43% identic al to a previously characterized chicken protein with homology to mammalian macrophage inflammatory protein 1 beta (MIP-1 beta), K203 exhibits about 5 0% sequence identity to human MIP-1 beta and other related CC chemokines. ( C) 2000 Academic Press.