The interferon-induced gene ISG12 is regulated by various cytokines as thegene 6-16 in human cell lines

The gene for ISG12 (originally designated p27) was isolated as an oestrogen -induced gene. The authors undertook a comprehensive study using quantitati ve RT-PCR, in which we delineate the regulation of ISG12 by seven different cytokines including interferons and poly(I). poly(C) in seven human cell l ines of different origin. In all cell lines ISG12 is strongly induced by IF N-alpha and only slightly by TFN gamma. Poly(I). poly(C) induces ISG12 in a cell line-dependent manner, whereas none of the other cytokines tested eli cited a response. Comparing the induction pattern of ISG12 to that of 6-16 a high degree of similarity was found. The induction levels varied, however , between cell lines. (C) 2000 Academic Press.