MsiK-dependent trehalose uptake in Streptomyces reticuli

During cultivation in the presence of trehalose Streptomyces reticuli expre sses an inducible. highly specific trehalose uptake system that is absent i n Streptomyces lividans. A palmitated trehalose-binding protein was identif ied in the cytoplasmic membrane of mycelia, extracted with the detergent Tr iton X-100 and purified using a trehalose affinity matrix. Immunological st udies showed that within S. reticuli the synthesis of the ATP-binding prote in MsiK is induced by trehalose. The data suggest that MsiK assists the tre halose ABC transporter, like the previously described ABC transport systems for maltose and cellobiose/cellotriose, respectively. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. A ll rights reserved.