Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli

Authors
Beard, SJ Hashim, R Wu, GH Binet, MRB Hughes, MN Poole, RK
Citation
Sj. Beard et al., Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli, FEMS MICROB, 184(2), 2000, pp. 231-235
Citations number
33
Categorie Soggetti
Microbiology
Journal title
FEMS MICROBIOLOGY LETTERS
ISSN journal
03781097 → ACNP
Volume
184
Issue
2
Year of publication
2000
Pages
231 - 235
Database
ISI
SICI code
0378-1097(20000315)184:2<231:EFTTOZ>2.0.ZU;2-A
Abstract
A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified through the generation of a zinc(II)-resistant mutant by transposon (Tn10d Cam) mutagenesis. The mutation was located within the pitA gene, which enco des the low-affinity inorganic phosphate transport system (Pit). The pitA m utant accumulated reduced amounts of zinc(II) when exposed to 0.5-2.0 mM Zn SO4 during growth in Luria-Bertani medium. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights re served.