Sj. Beard et al., Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli, FEMS MICROB, 184(2), 2000, pp. 231-235
A locus involved in zinc(II) uptake in Escherichia coli K-12 was identified
through the generation of a zinc(II)-resistant mutant by transposon (Tn10d
Cam) mutagenesis. The mutation was located within the pitA gene, which enco
des the low-affinity inorganic phosphate transport system (Pit). The pitA m
utant accumulated reduced amounts of zinc(II) when exposed to 0.5-2.0 mM Zn
SO4 during growth in Luria-Bertani medium. (C) 2000 Federation of European
Microbiological Societies. Published by Elsevier Science B.V. All rights re
served.