Production of N-acyl-L-homoserine lactones by P-aeruginosa isolates from chronic lung infections associated with cystic fibrosis

The N-acyl-L-homoserine lactones (AHLs) produced by sequential Pseudomonas aeruginosa isolates from chronically infected patients with cystic fibrosis a ere analyzed by thin-layer chromatography. It is demonstrated that both the amounts and the types of molecules synthesized by isolates from patient s who were monitored over periods of up to 11 years do not change significa ntly during chronic colonization. However: in the case of a patient who bec ame co-infected with an AHL-producing Burkholderia cepacia strain a dramati c reduction in the amounts of AHLs produced by the co-residing P. aeruginos a isolates was observed. (C) 2000 Published by Elsevier Science B.V. All ri ghts reserved.