NUCLEOTIDE-SEQUENCE AND ANALYSIS OF THE PHOB-RRNE-GROESL REGION OF THE BACILLUS-SUBTILIS CHROMOSOME

A 36 kb sequence of the phoB-rrnE-groESL region of the Bacillus subtil is chromosome at around 55 degrees has been determined. The sequenced region contains 36 ORFs including the phoB and groESL genes, and the w hole rrnE operon. The phoB gene is transcribed in the direction opposi te to that of chromosome replication, while most ORFs, including groES L and the rrnE operon, are transcribed in the same direction. Two newl y identified tRNA genes upstream of the rrnE operon were those for Arg -tRNA and Gly-tRNA. The sequenced region contains an operon consisting of genes for degradation and uptake of mannan. The rrnE operon and it s downstream ORFs are well conserved among Mycoplasma genitalium, Haem ophilus influenzae, Synechocystis so. and Methanococcus jannaschii. si gma(H) consensus sequences are present in the promoter regions of thre e ORFs, including groESL.