Sf. Chavante et al., A novel heparan sulphate with high degree of N-sulphation and high heparincofactor-II activity from the brine shrimp Artemia franciscana, INT J BIO M, 27(1), 2000, pp. 49-57
Citations number
34
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
With the aid of heparinase and heparitinases from Flavobacterium heparinum
and C-13 and H-I NMR spectroscopy it was shown that the heparan sulphate is
olated from the brine shrimp Artemia franciscana exhibits structural featur
es intermediate between those of mammalian heparins and heparan sulphates.
These include an unusually high degree of N-sulphation with corresponding v
ery low degree of N-acetylation), a relatively high content of iduronic aci
d residues (both unsulphated and 2-O-sulphated) and a relatively low degree
of 6-O-sulphation of the glucosamine residues. The major sequences (glucur
onic acid-->N-sulphated glucosamine and glucuronic acid--> N,6-disulphated
glucosamine) are most probably arranged in blocks. Although exhibiting negl
igible anticlotting activity in the APTT and anti-factor Xa assays the A. f
ranciscana heparan sulphate has a high heparin cofactor-II activity (about
1/3 that of heparin). (C) 2000 Elsevier Science B.V. All rights reserved.