Specific chaperone-like activity of inhibitor of caspase-activated DNase for caspase-activated DNase

Caspase-activated DNase (CAD) is the enzyme that causes DNA fragmentation d uring apoptosis. CAD forms aggregates when it is synthesized in the absence of an inhibitor of CAD (ICAD). Here, using renaturation systems of chemica lly denatured CAD, we report that ICAD-L, a long form of ICAD, has a chaper one-like activity specific for CAD. Murine CAD carries 14 cysteines, most o f which were found to be in reduced form. Reducing agents enhanced the prod uction of the functional CAD in an in vitro translation system, The denatur ed CAD could be efficiently renatured under highly reducing conditions only in the presence of ICAD-L. This process was ATP-independent. In contrast, reticulocyte lysates stimulated ICAD-L- and ATP-dependent renaturation of d enatured CAD without requiring a high concentration of reducing agents. The se results indicate that ICAD-L works not only as a specific inhibitor but also as a specific chaperone for CAD.