SINGLE-STEP IMMUNOAFFINITY PURIFICATION OF THE NEUROPEPTIDE SERICOTROPIN USING POLYCLONAL ANTIBODIES TOWARDS THE SYNTHETIC N-TERMINAL FRAGMENT OF THE MOLECULE

Authors
KODRIK D BERGHMAN L DELOOF A
Citation
D. Kodrik et al., SINGLE-STEP IMMUNOAFFINITY PURIFICATION OF THE NEUROPEPTIDE SERICOTROPIN USING POLYCLONAL ANTIBODIES TOWARDS THE SYNTHETIC N-TERMINAL FRAGMENT OF THE MOLECULE, European journal of entomology, 94(2), 1997, pp. 307-309
Citations number
8
Categorie Soggetti
Entomology
Journal title
European journal of entomology → ACNP
ISSN journal
12105759
Volume
94
Issue
2
Year of publication
1997
Pages
307 - 309
Database
ISI
SICI code
1210-5759(1997)94:2<307:SIPOTN>2.0.ZU;2-3
Abstract
An immunoaffinity chromatography of the neuropeptide sericotropin usin g rabbit polyclonal antibodies towards the 16 amino acids synthetic fr agment of the molecule was used for the purification of the peptide fr om Galleria mellonella larval brains. The method employed CNBr-activat ed Sepharose and the isolated IgG fraction of the corresponding antise rum. The purity of the peptide was proven by means of HPLC and immunob lotting techniques.