STR HUMARA locus gene and genotype frequencies in Han and Bei populations in China

For the purpose of the population generics study of the HUMARA locus, the a llele, and genotype frequencies were determined in two Chinese population s amples (Han-101, Bei-113) using PCR, PAGE, and silver staining. Fourteen al leles were found. The size of amplified fragments were 258 bp-315 bp. The o bserved heterozygosities were 0.83 in the Han population and 0.73 in the Be i population respectively. The expected heterozygosities were 0.91 in the H an population and 0.97 in the Bei population respectively. Both populations meet Hardy-Weinberg expectation, Han population x(2) = 17.7206, df = ii, p > 0.05; Bei population x(2) = 7.4268, df = 10, p > 0.05. The discriminatio n power were 0.95 in females and 0.89 in males in the Han population, 0.94 in females and 0.88 in males in the Bei population. Thus, the allelic frequ ency data can be used in the personal identification and parentage testing in the forensic science practice. The PCR test established in this study is robust and reproducible.