Analysis of antibody response to Cryptococcus neoformans in five patients with AIDS and cryptococcosis by immunoblotting

Citation
T. Lamura et al., Analysis of antibody response to Cryptococcus neoformans in five patients with AIDS and cryptococcosis by immunoblotting, J INFECTION, 40(1), 2000, pp. 64-68
Citations number
16
Categorie Soggetti
Immunology
Journal title
JOURNAL OF INFECTION
ISSN journal
01634453 → ACNP
Volume
40
Issue
1
Year of publication
2000
Pages
64 - 68
Database
ISI
SICI code
0163-4453(200001)40:1<64:AOARTC>2.0.ZU;2-X
Abstract
Objectives: The serological response of five patients with AIDS and cryptoc occosis to non capsular antigens from Cryptococcus neoformans var. neoforma ns has been investigated, Methods: Pressates of different isolates of C. neoformans were used as anti genic preparation for immunoblotting of patient samples, Results: Multiple sera and cerebrospinal fluids sequentially collected from five AIDS patients with cryptococcosis showed a wide heterogeneity in anti body response with bands at 48, 43, 38 and 26 kD being present in all clini cal samples of all five patients. The variation in handing patterns of the sequential samples from three patients was correlated with a decrease of th e antigen titre and with an amelioration of the cryptococcal infection, Conclusions: We identified antibodies to four immunodominant non-capsular a ntigens, which might represent major target molecules of the humoral respon se of patients with cryptococcosis. (C) 2000 The British Infection Society.