Evaluation of mini-VIDAS rapid test for detection of Listeria monocytogenes from production lines of fresh to cold-smoked fish

This study was conducted to evaluate the efficacy of the mini-VIDAS Listeri a rnonocytogenes (LMO) system (BioMerieux Vitek, Inc., Missouri, USA) for d etection of L.. monocytogenes in environmental and fish samples from three Portuguese cold-smoking plants and from their fresh fish suppliers. Mini-VI DAS-LMO is a fully automated system that uses fluorescent ELFA (Enzyme Link ed Fluorescent Assay) technology for detection of Listeria monocytogenes an tigens in food. It can be a rapid screening method alternative to time cons uming classical isolation and identification. Two hundred and ninety five s amples were tested in mini-VIDAS-LMO and in parallel by the ISO 11290-1 tra ditional protocol. The mini-VIDAS-LMO detected 8 of the 11 confirmed positi ve samples and presented 11 false positive results. The specificity of the mini-VIDAS-LMO found in this experiment was 0.96 and the sensitivity 0.73. (C) 2000 Elsevier Science B.V. All rights reserved.