Regulation of mouse delta-opioid receptor gene transcription: Involvement of the transcription factors AP-1 and AP-2

The aim of this study was to examine the effects of the phorbol ester O-tet radecanoylphorbol 13-acetate (TPA) and forskolin on delta-opioid receptor g ene transcription. Treatment of NG108-15 cells with TPA (100 nM) for 48 h i ncreased delta-opioid receptor mRNA levels, whereas different concentration s of forskolin induced a transient down-regulation of mRNA 5 h after treatm ent, followed by increased mRNA levels after 48 h. Reporter gene assays in transiently transfected NG108-15 cells in combination with electrophoretic mobility shift assays indicate that the increase of delta-opioid receptor m RNA after stimulation with TPA is mediated by transcription factor AP-1, wh ich binds 355 bp upstream of the start codon within the gene promoter. The forskolin-induced mRNA increase is mediated neither by a cyclic AMP-respons e element nor indirectly by AP-1 up-regulation. Reporter gene assays, mutat ional analysis, and electrophoretic mobility shift assays revealed that del ta-opioid receptor gene regulation by forskolin is mediated by transcriptio n factor AP-2, which binds to an element 157 bp upstream of the start codon .