Off-line analysis of red blood cell velocity in renal arterioles

Videomicroscopic methods with off-line analysis of microcirculatory paramet ers by multifunctional computer-assisted image analysis systems have signif icant advantages for in vivo microvascular research. A limitation of these methods is, however, that red blood cell velocities (V-RBC) exceeding 2 mm/ s cannot be measured using standard video framing rates, In the present stu dy, a high-speed video camera, recording up to 600 frames per seco nd, was incorporated in the set-up, and VRBC was measured off-line with the line-sh ift-diagram method. The aim of this study was to test the reproducibility a nd validity of the method using a high-speed video camera and to evaluate i ts applicability in vivo, VRBC were measured in arterioles of the split hyd ronephrotic kidney. The intra- and interindividual variability was small fo r V-RBC below 40 mm/s. The validity of the method was tested using the mass conservation principle and found to be at least as good as that of the dua l-slit photometric technique. The present approach extends the application of videomicroscopy coupled to image analysis systems to the analysis of hig h VRBC Copyright (C) 2000 S.Karger AG, Basel.