Effect of oral micronized purified flavonoid fraction treatment on leukocyte adhesion molecule expression in patients with chronic venous disease: A pilot study

Purpose: The purpose of this study was to determine the effects of a micron ized purified flavonoid fraction treatment on surface expression of leukocy te adhesion molecules in chronic venous disease (CVD), Methods: Twenty patients with chronic venous disease were assessed with the use of clinical and Duplex scanning criteria. Consenting patients were tre ated for 60 days with a micronized purified flavonoid fraction treatment (5 00 mg twice daily). Blood was collected from a foot vein immediately before the start of treatment and within 1 week after the treatment was stopped. Neutrophil and monocyte surface adhesion molecule expression was determined by flow cytometry using the monoclonal antibodies to CD11b and CD62L. Results: Neutrophil CD11b (248:212), monocyte CD11B (204:190), neutrophil C D62L (130:97 [P = .002]), and monocyte CD62L (170:121 [P = .03]) were deter mined, respectively, before and after treatment. All values are arbitrary u nits and represent median values, Conclusion Micronized purified flavonoid fraction treatment for 60 days sce nts to decrease the surface expression of CD62L by neutrophils and by monoc ytes. The clinical significance of this finding needs to be explores furthe r, It is feasible to use changes in the levels of these molecules as a mark er for response to therapy in chronic venous disease.