Citation
A. Mayr et al., Adaptation of reptilian paramyxovirus to mammalian cells (Vero cells), J VET MED B, 47(2), 2000, pp. 95-98
Citations number
8
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARYPUBLIC HEALTH
ISSN journal
09311793
→ ACNP
Volume
47
Issue
2
Year of publication
2000
Pages
95 - 98
Database
ISI
SICI code
0931-1793(200003)47:2<95:AORPTM>2.0.ZU;2-N
Abstract
The reptilian paramyxovirus GOV was successfully adapted to Vero cells in 8
0 passages at 30 degrees C. The virus replicated with HA titres of 1:64 and
10(7.5) TCID50/ml in the heterologous host cells forming syncytia, giant c
ells and cytoplasmic inclusion bodies. After 80 passages GOV was identified
by immunofluorescence, by immune electron microscopy and by PCR. Sequencin
g of RT-PCR products of GOV after 80 passages did not reveal changes in the
nucleotide sequence of the partial L-gene of GOV.