HLA class II alleles associations of anticardiolipin and anti-beta(2)GPI antibodies in a large series of European patients with systemic lupus erythematosus
M. Galeazzi et al., HLA class II alleles associations of anticardiolipin and anti-beta(2)GPI antibodies in a large series of European patients with systemic lupus erythematosus, LUPUS, 9(1), 2000, pp. 47-55
The objective of this study was to determine the HLA class II associations
of the anticardiolipin (aCL) and anti-beta(2)GPI (a beta(2)GPI) antibodies
in a large series of European patients with systemic lupus erythematosus (S
LE).
A cohort of 577 European SLE patients was enrolled. aCL and a beta(2)GPI we
re measured by ELISA methods. Molecular typing of HLA-DRB1, DRB3, DRB4, DRB
5, DQA1 and DQB1 loci was performed by the polymerase chain reaction-sequen
ce specific oligonucleotide probes (PCR-SSOP) method.
aCL of IgG, IgM and IgA isotypes were detected in 22.8%, 14% and 13.9% of p
atients, respectively. IgG and IgM a beta(2)GPI were detected in 20% of pat
ients, aCL showed positive association with HLA DRB1*04, DRB1*0402, DRB1*04
03, DRBI*07, DRB3*0301, DQA1*0201, DQA1*0301, DQB1*0302, and negative assoc
iation with DQA1*0501, DRB3*0202. a beta(2)GPI showed positive association
with DRB1*0402, DRB1*0403, DQB1*0302. DRB1*0402 carried the highest relativ
e risk for the presence of both aCL (RR = 8.1) and a beta(2)GPI (RR = 4.6).
Our results confirm the already described associations of aCL with HLA DR4
and DR7, but also demonstrate that, among the alleles at the DRB1*04 locus;
the *0402 was most represented both in aCL and in a beta(2)GPI positive pa
tients. In addition, HLA class II associations of a beta(2)GPI are for the
first time extensively examined in a large cohort of European SLE patients.