Plasmid instability in methylotrophic yeast Hansenula polymorpha: The capture of chromosomal DNA fragments by replicative plasmids

Authors
Bogdanova, AI Agaphonov, MO Ter-Avanesyan, MD
Citation
Ai. Bogdanova et al., Plasmid instability in methylotrophic yeast Hansenula polymorpha: The capture of chromosomal DNA fragments by replicative plasmids, MOL BIOL, 34(1), 2000, pp. 29-32
Citations number
23
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
34
Issue
1
Year of publication
2000
Pages
29 - 32
Database
ISI
SICI code
0026-8933(200001/02)34:1<29:PIIMYH>2.0.ZU;2-7
Abstract
In Hansenula polymorpha, integrative plasmids become replicative as a resul t of capturing chromosomal fragments that contain HARS providing for their autonomous replication. A similar structural instability was observed at a low frequency in H. polymorpha replicative vectors. Most of the fragments c aptured acted as promoters and sustained autonomous replication.