Affinity separation and selective modification of HeLa chromatin proteins associated with GT repeats

Authors
Knorre, DG Bozhenok, LN Chernolovskaya, EL Shishkina, IG Godovikova, TS Zarytova, VF Kobets, ND
Citation
Dg. Knorre et al., Affinity separation and selective modification of HeLa chromatin proteins associated with GT repeats, MOL BIOL, 34(1), 2000, pp. 45-48
Citations number
16
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
34
Issue
1
Year of publication
2000
Pages
45 - 48
Database
ISI
SICI code
0026-8933(200001/02)34:1<45:ASASMO>2.0.ZU;2-8
Abstract
Affinity sorption of the HeLa nuclear protein on d(pGpT)(6), d(pApC)(6), or their duplex immobilized on a matrix revealed several proteins, some bindi ng with the duplex only. Sorption of some proteins was suppressed by an equ ivalent amount of d(pApC)(6), suggesting their general affinity for single- stranded oligonucleotides rather than specific binding. Treatment of cell n uclei with photoreactive d(pGpT)(6) resulted in modification of seven chrom atin proteins. Of these, only a 67K protein specifically bound with all sor bents.