The second methyltransferase of the BstF5I restriction-modification systemis homologous to the C-terminal domains of FokI and StsI methylases

Authors
Abdurashitov, MA Netesova, NA Golikova, LN Gutorov, VV Belavin, PA Degtyarev, SK
Citation
Ma. Abdurashitov et al., The second methyltransferase of the BstF5I restriction-modification systemis homologous to the C-terminal domains of FokI and StsI methylases, MOL BIOL, 34(1), 2000, pp. 74-80
Citations number
13
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR BIOLOGY
ISSN journal
00268933 → ACNP
Volume
34
Issue
1
Year of publication
2000
Pages
74 - 80
Database
ISI
SICI code
0026-8933(200001/02)34:1<74:TSMOTB>2.0.ZU;2-D
Abstract
The bstF5IM-2 gene for the second DNA methyltransferase of Bacillus stearot hermophilus F5 (M.BstF5I-2) was cloned and sequenced. On the chromosome, th e gene is located downstream of the gene for M.BstF5I-1 and is oriented sim ilarly. The protein encoded has conserved regions specific for adenine meth ylases of subclass D-12. M.BstF5I-2 is homologous to the C-terminal domains of M.FokI and M.StsI and recognizes the same sites. M.BstF5I-1 modifies th e DNA upper strand and M.BstF5I-2 modifies the other strand in the recognit ion site.