Bone morphogenetic protein receptor complexes on the surface of live cells: A new oligomerization mode for serine/threonine kinase receptors

The bone morphogenetic proteins (BMPs) play important roles in embryogenesi s and normal cell growth. The BMP receptors belong to the family of serine/ threonine kinase receptors, whose activation has been investigated intensiv ely for the transforming growth factor-beta (TGF-beta) receptor subfamily. However, the interactions between the BMP receptors, the composition of the active receptor complex, and the role of the ligand in its formation have not yet been investigated and were usually assumed to follow the same patte rn as the TGF-beta receptors. Here we demonstrate that the oligomerization pattern of the BMP receptors is different and is more flexible and suscepti ble to modulation by ligand. Using several complementary approaches, we inv estigated the formation of homomeric and heteromeric complexes between the two known BMP type I receptors (BR-Ia and BR-Ib) and the BMP type II recept or (BR-II). Coimmunoprecipitation studies detected the formation of heterom eric and homomeric complexes among all the BMP receptor types even in the a bsence of ligand. These complexes were also detected at the cell surface af ter BMP-2 binding and cross-linking. Using antibody-mediated immunofluoresc ence copatching of epitope-tagged receptors, we provide evidence in live ce lls for preexisting heteromeric (BR-II/ BR-Ia and BR-II/BR-Ib) and homomeri c (BR-II/BR-II, BR-Ia/BR-Ia, BR-Ib/BR-Ib, and also BR-Ia/BR-Ib) oligomers i n the absence of ligand. BMP-2 binding significantly increased hetero- and homo-oligomerization (except for the BR-II homo-oligomer, which binds ligan d poorly in the absence of BR-I). In contrast to previous observations on T GF-beta receptors, which were found to be fully homodimeric in the absence of ligand, the BMP receptors show a much more flexible oligomerization patt ern. This novel feature in the oligomerization mode of the BMP receptors al lows higher variety and flexibility in their responses to various ligands a s compared with the TGF-beta receptors.