A comparative study of a liquid and a solid matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and collision cross section measurements
S. Ring et Y. Rudich, A comparative study of a liquid and a solid matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and collision cross section measurements, RAP C MASS, 14(6), 2000, pp. 515-519
We present experimental matrix-assisted laser desorption/ionization time-of
-flight (MALDI-TOF) results comparing a liquid (glycerol/K-4[Fe(CN)(6)]) an
d a solid matrix (2,5-dihydroxybenzoic acid, DHB) with respect to analyte s
ignal stability and initial ion velocity. For applications requiring stable
production of analyte ions over a long period of time, the liquid matrix i
s superior to the solid matrix. The stable analyte ion signal obtained from
a liquid matrix allowed the measurement of collision cross sections of sma
ll poly(ethylene glycol) (PEG(n)) adduct ions in the flight tube with good
resolution. The initial velocity of these adduct ions was measured. It was
found that analyte molecules from the liquid matrix have initial ion veloci
ties significantly smaller than those from the solid matrix. MALDI-TOF meas
urements for large molecules using a liquid matrix are therefore likely to
result in smaller systematic errors in mass calibrations due to initial ion
velocity. Copyright (C) 2000 John Wiley & Sons, Ltd.