Determination of a new asiatic acid derivative, AS 2-006A in rat plasma and urine, and human plsma by high-performance liquid chromatography

A high-performance liquid chromatographic method was developed for the dete rmination of a new wound healing agent, AS 2-006A (ethoxymethyl 2-oxo-3, 23 -O-isopropylideneasiatate), in rat plasma and urine, and human plasma. The sample preparation was simple: 2 volumes of acetonitrile were added to the biological samples to deproteinize it. A 50-mu l aliquot of the supernatant was injected onto the reversed-phase column. The mobile phase employed was acctonitrile : H2O (9:1, v/v) and run at a flow rate of 1.1 ml/min. The co lumn effluent was monitored by a UV detector set at 205 nm. The retention t ime for AS 2-006A was approximately 29.5 min. The detection limits for AS 2 -006A in rat and human plasma were both 1 mu g/ml, and in rat urine was 2 m u g/ml. The coefficients of variation of the assay (within-day and between- day) were generally low (below 10.8%) for rat plasma and urine, and human p lasma. No interferences from endogenous substances were found.