From sequence to chromosome: The tip of the X chromosome of D. melanogaster

Authors
Benos, PV Gatt, MK Ashburner, M Murphy, L Harris, D Barrell, B Ferraz, C Vidal, S Brun, C Demailles, J Cadieu, E Dreano, S Gloux, S Lelaure, V Mottier, S Galibert, F Borkova, D Minana, B Kafatos, FC Louis, C Siden-Kiamos, I Bolshakov, S Papagiannakis, G Spanos, L Cox, S Madueno, E de Pablos, B Modolell, J Peter, A Schottler, P Werner, M Mourkioti, F Beinert, N Dowe, G Schafer, U Jackle, H Bucheton, A Callister, DM Campbell, LA Darlamitsou, A Henderson, NS McMillan, PJ Salles, C Tait, EA Valenti, P Saunders, RDC Glover, DM
Citation
Pv. Benos et al., From sequence to chromosome: The tip of the X chromosome of D. melanogaster, SCIENCE, 287(5461), 2000, pp. 2220-2222
Citations number
32
Categorie Soggetti
Multidisciplinary,Multidisciplinary,Multidisciplinary
Journal title
SCIENCE
ISSN journal
00368075 → ACNP
Volume
287
Issue
5461
Year of publication
2000
Pages
2220 - 2222
Database
ISI
SICI code
0036-8075(20000324)287:5461<2220:FSTCTT>2.0.ZU;2-I
Abstract
One of the rewards of having a Drosophila melanogaster whole-genome sequenc e will be the potential to understand the molecular bases for structural fe atures of chromosomes that have been a Long-standing puzzle. Analysis of 2. 6 megabases of sequence from the tip of the X chromosome of Drosophila iden tifies 273 genes. Cloned DNAs from the characteristic bulbous structure at the tip of the X chromosome in the region of the broad complex display an u nusual pattern of in situ hybridization. Sequence analysis revealed that th is region comprises 154 kilobases of DNA flanked by 1.2-kilobases of invert ed repeats, each composed of a 350-base pair satellite related element. Thu s, some aspects of chromosome structure appear to be revealed directly with in the DNA sequence itself.