Metabolism and disposition of luminol in the rat

1. The metabolism and disposition of Luminol (LMN. 3-aminophthalhydrazide), a widely used forensic and laboratory reagent that chemiluminesses upon ox idation, was determined as part of its overall toxicological characterizati on. 2. Radiolabelled LMN was well absorbed, metabolized and excreted following p.o. administration of a range of doses. About 90 % of the total dose was r ecovered within 21 h of administration in urine in the form of two metaboli tes identified as LMN N-8-glucuronide and LMN N-8-sulphamic acid. 3-Aminoph thalic acid, the oxidative product of LMN in the light-emitting reaction, w as apparently not formed in vivo. 3. Metabolism and disposition of an i.v. administered dose was similar to t hat following gavage. Little or no LMN-derived radioactivity was present in tissue within 12 h postdosing. Excretion of radioactivity in bile followin g i.v. injection was minimal (similar to 8 % of the total dose in 6 h) and consisted of the same urinary-excreted glucuronide and sulphate conjugates. 4. LMN was not absorbed dermally in rat, potentially a major route of expos ure to human. If the fate of LMN is similar between species, this compound should have little potential for either dermal absorption, bioaccumulation in tissues following other routes of exposure or chronic toxicity in humans .