Expression of beta ig-h3 by human bronchial smooth muscle cells localization to the extracellular matrix and nucleus

Bronchial smooth muscle cells play a central role in normal lung physiology by controlling airway tone. In addition, airway smooth muscle hyperplasia and hypertrophy are important factors in the pathophysiology of asthma. In this study, expression of beta ig-h3, a recently identified component of th e extracellular matrix (ECM), was investigated in primary human bronchial s mooth muscle (HBSM) cells, Northern blot analysis demonstrated that treatme nt of cultured HBSM cells with transforming growth factor-beta 1 resulted i n a 4- to 5-fold increase in the steady-state level of beta ig-h3 messenger RNA. Western blot analysis of secreted proteins using monospecific antibod ies generated against peptide sequences found in the N- and C-terminal regi ons of the protein identified several isoforms having apparent mass of 70-7 4 kD. Immunohistochemical analysis of human lung localized beta ig-h3 to th e vascular and airway ECM, and particularly to the septal tips of alveolar ducts and alveoli, suggesting that it may have a morphogenetic role. Analys is of cultured HBSM cells identified beta ig-h3 in both the ECM as well as the cytoplasm, and surprisingly also in the nucleus. These results demonstr ate that beta ig-h3 is produced by resident lung cells, is a component of l ung ECM, and may play an important role in lung structure and function. The presence of this protein in nuclei suggests that it may have regulatory fu nctions in addition to its role as a structural component of lung ECM.