Separation and determination of niobium(V) and tantalum(V) as 2-(5-bromo-2-pyridylazo)-5-[N-propyl-N-(3-sulfopropyl)amino] phenol citrate ternary complexes in geological samples using ion interaction high-performance liquid chromatography

A method for the simultaneous separation and determination of Nb(V) and Ta( V) as ternary complexes formed with 2-(5-bromo-2-pyridylazo)-5-[N-propyl-N- (3-sulfoproyl)amino]phenol (PAPS) and citrate was developed using ion inter action reversed-phase high-performance liquid chromatography (HPLC) on a Cl s column. An artificial neural network (ANN) in combination with a reduced factorial experimental design was utilised to optimise the mobile phase com positions and the conditions used for pre-column complex formation. Under t he optimum conditions determined by the ANN, the Nb(V) and Ta(V) complexes were eluted within 5 min. The detection limits (signal to noise ratio = 3, detection wavelength 600 nm) for Nb(V) and Ta(V) were 0.03 and 0.40 mu g/l, respectively, which correspond to 0.03 and 0.40 mu g/g in the solid sample s. The method was applied to the analysis of geological samples and the res ults obtained by the HPLC method agreed well with the results obtained by I CP-MS and also with the certified values. (C) 2000 Elsevier Science B.V. Al l rights reserved.