C. Abeygunawardana et al., Development and validation of an NMR-based identity assay for bacterial polysaccharides, ANALYT BIOC, 279(2), 2000, pp. 226-240
A method utilizing NMR spectroscopy has been developed to confirm the ident
ity of bacterial polysaccharides used to formulate a polyvalent pneumococca
l polysaccharide vaccine. The method is based on 600 MHz proton NMR spectra
of individual serotype-specific polysaccharides. A portion of the anomeric
region of each spectrum (5.89 to 4.64 ppm) is compared to spectra generate
d for designated reference samples for each polysaccharide of interest. The
selected region offers a spectral window that is unique to a given polysac
charide and is sensitive to any structural alteration of the repealing unit
s. The similarity of any two spectral profiles is evaluated using a correla
tion coefficient (rho), where rho greater than or equal to 0.95 between a s
ample and reference profile indicates a positive identification of the samp
le polysaccharide. This method has been shown to be extremely selective in
its ability to discriminate between serotype-specific polysaccharides, some
of which differ by no more than a single glycosidic linkage. Furthermore,
the method is rapid and does not require extensive sample manipulations or
pretreatments. The method was validated as a qualitative identity assay and
will be incorporated into routine quality control testing of polysaccharid
e powders to be used in preparation of the polyvalent pneumococcal vaccine
PNEUMOVAX 23. The specificity and reproducibility of the NMR-based identity
assay is superior to the currently used colorimetric assays and can be rea
dily adapted for use with other bacterial polysaccharide preparations as we
ll. (C) 2000 Academic Press.