Nigh-temperature ultrafast liquid chromatography

A novel liquid chromatographic system which enables high temperature ultraf ast liquid chromatography (HTU-FLC) has been designed through the careful c onsideration of heat transfer, band broadening, and pressure drop. Studies of the effect of linear velocity on the HETP show that column efficiency at high velocity, especially of well-retained solutes, dramatically improves at higher temperatures. At 150 degrees C, at a flow rate of 15 mL/min with a 5 cm by 4.6 mm (i.d.) column packed with 3 mu m polystyrene-coated zircon ia porous particles, long chain alkylphenones were completely resolved, and the analysis time could be decreased by a factor of 50 compared to that at room temperature (25 degrees C) at a conventional flow rate (4 mL/min). In addition, using pure water as the mobile phase, five phenols were separate d in less than 30 s.