Structural organization of the human glutathione reductase gene: Determination of correct cDNA sequence and identification of a mitochondrial leader sequence

The primary structure of human glutathione reductase gene (GSR) was determi ned by genomic cloning. The gene structure of human GSR spans 50 kb, consis ts of 13 exons, and was found to be highly similar to the mouse GSR gene. T he coding sequence of human GSR resides on all 13 exons. An N-terminal argi nine-rich mitochondrial leader sequence was present, with high homology to the murine leader sequence, between two in-frame start codons in the first exon. The 5' and 3' intron/exon splice junctions, with one exception, follo wed the general consensus sequences for intron spliced donor and acceptance sites. (C) 2000 Academic Press.