Cytochrome P450 1A2: Oligomers in proteoliposomes

The presence of oligomers of cytochrome P450 1A2 in membranes of proteolipo somes produced by the cholate-dialysis technique was demonstrated by cross- linking of protein molecules with bifunctional reagents followed by electro phoretic analysis of the modified proteins. A hexameric organization of cyt ochrome P450 1A2 in the membrane of proteoliposomes is suggested with high probability based on the comparison of the purified hemoprotein oligomeric structure in an aqueous medium and that in the proteoliposomes. The compari son was carried out using the same method.