Isolation and characterization of soybean Bowman-Birk inhibitor from different sources

A chromatographic procedure for isolation of different isoforms of Bowman-B irk soybean trypsin inhibitors was developed. The number of isoforms was sh own to depend on soybean cultivar. The amount of the classical Bowman-Birk inhibitor (BBI) in different soybean cultivars, commercial flour, and proce ssing products was analyzed. BBI reaches its highest concentration in fresh ly milled seeds. Storage conditions optimum for preservation of maximum inh ibitory activity in soybean raw material were developed. The use of indirec t enzyme immunoassay for BBI detection during its isolation from different sources was demonstrated.