Mixed-feed exponential feeding for fed-batch culture of recombinant methylotrophic yeast

A simple, accurate model capable of predicting cell growth and methanol uti lization during the mixed substrate fed-batch fermentation of Mut(S) recomb inant Pichia pastoris was developed and was used to design an exponential f eeding strategy for mixed substrate fed-batch fermentation at a constant sp ecific growth rate. Mixed substrate feeding has been shown to boost product ivity in recombinant fed-batch culture of P. pastoris, while fixed growth r ate exponential feeding during fed-batch culture is a useful tool in proces s optimization and control.