The distribution of Ca2+ in the anthers of wheat was observed using cytoche
mical method of potassium antimonite. At the later tetrad stage, Ubisch bod
ies carrying Ca2+ were observed on the inner surface of tapetum, in anther
locule and on pollen surface. The Ubisch bodies contacted with pollen, and
Ca2+ began lo accumulate on pollen surface. At the uninucleate pollen stage
, abundant Ubisch bodies were distributed in anther locule, and the amount
of Ca2+ on pollen surface increased. At the mature pollen stage a large amo
unt of Ca2+ ions were localized on the inner surface of tapetum, the surfac
e of pollen and Ubisch bodies. In the pollen wall, Ca2+ precipitates arrang
ed in radial lines. These results demonstrated that Ubisch bodies were invo
lved in Ca2+ transport from anther wall to pollen surface at some developme
ntal stages of anther.